Content

Publications

2017

Goetzke R, Franzen J, Ostrowska A, Vogt M, Blaeser A, Klein G, Rath B, Fischer H, Zenke M, Wagner W. Does Soft Really Matter? Differentiation of Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells is Not Influenced by Soft Hydrogels. Biomaterials, Epub ahead of print.

Frobel J, Bozic T, Lenz M, Uciechowski P, Han Y, Herwartz R, Strathmann K, Isfort S, Panse J, Esser A, Birkhofer C, Gerstenmaier U, Kraus T, Rink L, Koschmieder S, Wagner W. Leukocyte counts based on DNA methylation at individual cytosines. Clinical Chemistry, Epub ahead of print.

Fernandez-Rebollo E, Eipel M, Seefried L, Hoffmann P, Strathmann, K, Jakob F*, Wagner W*. Primary osteoporosis is not reflected by disease-specific DNA. Journal of Bone and Mineral Research, Epub ahead of print.

Abagnale G, Sechi A, Steger M, Zhou Y, Kuo CC, Aydin G, Schalla C, Müller-Newen G, Zenke M, Costa IG, van Rijn P, Gillner A, Wagner W. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies. Stem Cell Reports, 2017, 9:654-666.

Ventura Ferreira MS, Crysandt M, Ziegler P, Hummel S, Wilop S, Kirschner M, Schemionek M, Jost E, Wagner W, Brümmendorf TH, Beier F. Evidence for a pre-existing telomere deficit in non-clonal hematopoietic stem cells in patients with acute myeloid leukemia. Annals of Hematology, 2017, 96: 1457-1461.

Fernandez-Rebollo E, Mentrup B, Ebert R, Franzen J, Abagnale G, Sieben T, Hoffmann P, Roux PF, Rath B, Goodhardt M, Lemaitre J-M, Bischof O, Franz J, Wagner W. Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells. Scientific Reports, 2017, 7: 5132.

Franzen J, Zirkel A, Blake J, Rath B, Benes V, Papantonis A, Wagner W. Senescence-Associated DNA Methylation is Stochastically Acquired in Subpopulations of Mesenchymal Stem Cells. Aging Cell, 2017, 16: 183-191.

Rose M, Kloten V, Noetzel E, Gola L, Ehling J, Heide T, Meurer  SK, Gaiko-Shcherbak A, Sechi AS, Huth S, Weiskirchen R, Klaas O, Antonopoulos W, Lin Q, Wagner W, Veeck J, Gremse F, Steitz J, Knüchel R, Dahl E. ITIH5 mediates epigenetic reprogramming of breast cancer cells. Molecular Cancer, 2017, 16:44.

Zhang Y, Hapala J, Brenner H, Wagner W. Individual CpG Sites that are Associated with Age and Life Expectancy become Hypomethylated upon Aging. Clinical Epigenetics, 2017, 9: 9.

Wagner W. Epigenetic aging clocks in mice and men. Genome Biology, 2017, 18: 107.

Moskalev A, Anisimov V, Aliper A, Artemov A, Asadullah K, Belsky D, Baranova A, de Grey A, Dixit VD, Debonneuil E, Dobrovolskaya E, Fedichev P, Fedintsev A, Fraifeld V, Franceschi C, Freer R, Fülöp T, Feige J, Gems D, Gladyshev V, Gorbunova V, Irincheeva I, Jager S, Jazwinski SM, Kaeberlein M, Kennedy B, Khaltourina D, Kovalchuk I, Kovalchuk O, Kozin S, Kulminski A, Lashmanova E, Lezhnina K, Liu GH, Longo V, Mamoshina P, Maslov A, Pedro de Magalhaes J, Mitchell J, Mitnitski A, Nikolsky Y, Ozerov I, Pasyukova E, Peregudova D, Popov V, Proshkina E, Putin E, Rogaev E, Rogina B, Schastnaya J, Seluanov A, Shaposhnikov M, Simm A, Skulachev V, Skulachev M, Solovev I, Spindler S, Stefanova N, Suh Y, Swick A, Tower J, Gudkov AV, Vijg J, Voronkov A, West M, Wagner W, Yashin A, Zemskaya N, Zhumadilov Z, Zhavoronkov A. A review of the biomedical innovations for healthy longevity. Aging (Albany NY). 2017,9: 7-25.

2016

Kalwa M, Hänzelmann S, Otto S, Kuo Chao-Chung, Franzen J, Joussen S, Fernandez Rebollo E, Rath B, Koch C, Hofmann A, Lee S-H, Teschendorff A, Denecke B, Lin Q, Widschwendter M, Weinhold E, Costa I, and Wagner W. The lncRNA HOTAIR Impacts on Mesenchymal Stem Cells via Triple Helix FormationNucleic Acid Research, 2016; 44: 10631-10643.

Wilop S, Chou W-C, Jost E, Crysandt M, Panse J, Chuang M-K, Brümmendorf T H, Wagner W, Tien H-F, Kharabi Masouleh B. A three-gene expression based risk score can refine the European LeukemiaNet AML classification. Journal of Hematology & Oncology, 2016; 9: 78.

Eipel M, Mayer F, Arent T, Ferreira MRP, Birkhofer C, Gerstenmaier U, Costa IG, Ritz-Timme S, Wagner W. Epigenetic age predictions based on buccal swabs are more precise in combination with cell type-specific DNA methylation signatures. Aging US, 8: 1034-1048.

Wagner W, Frobel J, Goetzke R. Epigenetic Quality Check – How Good are Your Mesenchymal Stromal Cells? Epigenomics, 2016; 8: 889-894.

Vasko T, Frobel J, Lubberich R, Goecke TW, Wagner W. iPSC-Derived Mesenchymal Stromal Cells are Less Supportive than Primary MSCs for Co-Culture of Hematopoietic Progenitor Cells. Journal of Hematology & Oncology, 2016. 9: 43. 

Franzen J, Wagner W, Fernandez-Rebollo E. Epigenetic Modifications upon Senescence of Mesenchymal Stem Cells. Current Stem Cell Reports, 2016; 2: 248–254.

Lin Q, Weidner CI, Costa I, Marioni R, Ferreira M, Deary I and Wagner W. DNA methylation levels at individual age-associated CpG sites can be indicative for life expectancy. Aging, 2016; 8: 394-401.

Weidner CI, Lin Q, Birkhofer C, Gerstenmaier U, Kaifie A, Kirschner M, Bruns H, Balabanov S, Trummer A, Stockklausner C, Höchsmann B, Schrezenmeier H, Wlodarski M, Panse J, Brümmendorf TH, Beier F, Wagner W. DNA methylation in PRDM8 is indicative for dyskeratosis congenita. Oncotarget, 2016. doi: 10.18632/oncotarget.7458.

Candio de Almeida D, Ferreira MRP, Franzen J, Weidner C, Frobel J, Zenke M, Costa IG, Wagner W. Epigenetic classification of human mesenchymal stromal cells. Stem Cell Reports, 2016, 6: 168-75.

Wagner W, Fernandez-Rebollo E, Frobel J. DNA-Methylation Changes in Replicative Senescence and Aging: Two Sides of the Same Coin? Epigenomics, 2016 8: 1-3.

Schulze I, Rohde C, Scheller M, Krause A, Herbst F, Riemke P, Hebestreit K, Tschanter P, Lin Q, Linhart H, Godley L, Glimm H, Dugas M, Wagner W, Bredel W, Rosenbauer R, Müller-Tidow C. Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis. Blood. 2016; 127: 1575-86.

2015

Božić T, Lin Q, Frobel J, Wilop S, Hoffmann M, Müller-Tidow C, Brümmendorf T H, Jost E, Wagner W. DNA-Methylation in C1R is a Prognostic Biomarker for Acute Myeloid Leukemia. Clinical Epigenetics. 2015, 7: 116.

Teschendorff AE, Jones A, Lee SH, Fiegl H, Kalwa M, Wagner W, Chindera K, Evans I, Dubeau L, Orjalo A, Horlings HM, Niederreiter L, Kaser A, Yang W, Goode EL, Fridley BL, Jenner RG, Berns EMJJ, Wik E, Salvesen HB, Wisman GBA, van der Zee AGJ, Davidson B, Trope CG, Lambrechts S, Vergote I, Calvert H, Jacobs IJ, Widschwendter M. HOTAIR and its Surrogate DNA Methylation Signature indicate Carboplatin Resistance in Ovarian Cancer. Genome Medicine 2015, 7: 108.

Diener Y, Walenda T, Jost E, Brümmendorf TH, Bosio A, Wagner W, Bissels U. MicroRNA expression profiles of serum from patients before and after chemotherapy. Genomics Data. 2015; 6: 125-127.

Erdmann G, Suchanek M, Horn P, Graf F, Volz C, Horn T, Zhang X, Wagner W, Ho AD, Boutros M. Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi Screening. Genome Medicine 2015, 7:46 

Lin Q, Wagner W. Epigenetic Aging Signatures are Coherently Modified in CancerPLoS Genetics 2015; 11: e1005334.

Abagnale G, Steger M, Nguyen VH, Hersch N, Sechi A, Joussen S, Denecke B, Merkel R, Hoffmann B, Dreser A, Schnakenberg U, Gillner A, Wagner W. Surface Topography Enhances Differentiation of Mesenchymal Stem Cells Towards Osteogenic and Adipogenic Lineages. Biomaterials 2015; 61: 316-26.

Hänzelmann S, Beier F, Gusmao EG, Koch CM, Hummel S, Charapitsa I, Joussen S, Benes B, Brümmendorf TH, Reid G, Costa IG, Wagner W. Replicative Senescence is Associated with Nuclear Reorganization and with DNA Methylation at Specific Transcription Factor Binding Sites. Clinical Epigenetics 2015; 7: 19.

Lenz M, Goetzke R, Schenk A, Schubert C, Veeck J, Hemeda H, Koschmieder S, Zenke M, Schuppert A, Wagner W. Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells. Scientific Reports 2015; 5: 8973. 

Steger M, Abagnale G, Bremus-Köbberling E, Wagner W, Gillner A. Nanoscale biofunctionalization of polymer surfaces by laser treatment for controlled cellular differentiation; in König/Ostendorf (Eds.), Optically Induced Nanostructures. Biomedical and Technical Applications, De Gruyter 2015; ISBN 978-3-11-038350-8, Berlin; 2015.

Walenda T, Diener Y, Jost E, Morin-Kensicki E, Goecke TW, Bosio A, Rath B, Brümmendorf TH, Bissels U, Wagner W. MicroRNAs and Metabolites in Serum Change after Chemotherapy: Impact on Hematopoietic Stem and Progenitor Cells. PLoS ONE 2015; 10: e0128231.

Weidner CI, Ziegler P, Hahn M, Brümmendorf TH, Ho AD, Dreger P, Wagner W. Epigenetic aging upon allogeneic transplantation: The hematopoietic niche does not affect age-associated DNA methylation. Leukemia 2015; 29: 985-8.

Reinisch A, Etchart N, Hofmann NA, Thomas D, Fruehwirth M, Sinha S, Hartwig UF, Beham-Schmid C, Trajanoski S, Lin Q, Wagner W, Dullin C, Alves F, Schallmoser K, Andreeff M, Strunk M. Epigenetic and in vivo comparison of diverse mesenchymal stromal cell sources reveals an endochondral signature for human hematopoietic niche formation. Blood 2015; 125: 249-60.

Wagner W, Weidner CI, Lin Q. Do age-associated DNA methylation changes increase the risk of malignant transformation? BioEssays, 2015, 37:20-4.

2014

Frobel J, Hemeda H, Lenz M, Abagnale G, Joussen S, Denecke B, Šarić T, Zenke M, Wagner W. Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells. Stem Cell Reports. 2014; 3: 414-422. http://www.cell.com/stem-cell-reports/abstract/S2213-6711(14)00233-1

Qin J, Sontag S, Lin Q, Mitzka S, Leisten I, Schneider RK, Wang X, Jauch A, Peitz M, Brüstle O, Wagner W, Zhao RC, Zenke M. Cell Fusion Enhances Mesendodermal Differentiation of Human Induced Pluripotent Stem Cells. Stem Cells Dev. 2014. (epub ahead of print).

Weidner CI, Wagner W. The Epigenetic Tracks of Aging. Biol. Chem. 2014; 395: 1307-1314.

Abnaof K, Mallela N, Walenda G, Meurer S, Seré K, Lin Q, Smeets B, Hoffmann K, Wagner W, Zenke M, Weiskirchen R, Fröhlich H. TGF-beta Stimulation in Human and Murine Cells Reveals Commonly Affected Biological Processes and Pathways at Transcription Level. BMC Systems Biology, 2014; 8: 55.

Schellenberg A, Joussen S, Moser K, Hampe N, Hersch N, Hemeda H, Schnitker J, Denecke B, Lin Q, Pallua N, Zenke M, Merkel R, Hoffmann B, Wagner W. Matrix Elasticity, Replicative Senescence and DNA Methylation Patterns of Mesenchymal Stem Cells. Biomaterials. 2014; 35: 6351.http://www.ncbi.nlm.nih.gov/pubmed/24824582

Schellenberg A, Mauen S, Koch CM, Jans R, de Waele P, Wagner W. Proof of Principle: Quality Control of Therapeutic Cell Preparations using Senescence-Associated DNA-Methylation Changes. BMC Research Notes, 2014; 23: 25.http://www.ncbi.nlm.nih.gov/pubmed/24824582

Wuchter P, Bieback K, Schrezenmeier H, Bornhäuser M, Müller LP, Bönig H, Wagner W, Meisel R, Paveli P, Tonn T, Lang P, Müller I, Renner M, Malchereka G, Saffrich R, Buss E, Horn P, Rojewskic M, Schmitt A, Ho AD, Sanzenbacher R, Schmitt M. Standardization of GMP-compliant production of bone marrow derived human mesenchymal stromal cells Cytotherapy,(epub ahead of print).

Walenda T*, Stiehl T*, Braun H, Fröbel J, Ho AD, Schroeder T, Goecke TW, Rath B, Germing U, Marciniak-Czochra A, Wagner W. Feedback Signals in Myelodysplastic Syndromes: Increased Self-Renewal of the Malignant Clone Suppresses Normal Hematopoiesis. PLoS Computational Biology 2014; 10: e1003599.

Weidner CI, Lin Q, Koch CM, Eisele L, Beier F, Ziegler P, Bauerschlag DO, Jöckel KH, Erbel R, Mühleisen TW, Zenke M, Brümmendorf TH, Wagner W. Aging of blood can be tracked by DNA methylation changes at just three CpG sites. Genome Biology, 2014; 15: R24.

Radtke S, Giebel B, Wagner W, Horn P. Platelet Lysates and their Role in Cell Therapy. ISBT Science Series, 2014 (epub ahead of print).

Schellenberg A, Ross R, Abagnale G, Joussen S, Schuster P, Arshi A, Pallua N, Jockenhoevel, S, Gries T, Wagner W. 3D Non-Woven Polyvinylidene Fluoride Scaffolds: Fibre Cross Section and Texturizing Patterns have Impact on Growth of Mesenchymal Stromal Cells. PLoS ONE, 2014; 9: e94353

Jost E, Lin Q, Weidner CI, Wilop S, Hoffmann M, Walenda T, Schemionek M, Herrmann O, Zenke M, Brümmendorf T, Koschmieder S., Wagner W. Epimutations Mimic Genomic Mutations of DNMT3A in Acute Myeloid Leukemia. Leukemia, 2014; 28: 1227-1234.

2013

Weidner CI, Walenda T, Lin Q, Wölfler MM, Denecke B, Gesteira Costa I, Zenke M, Wagner W. Hematopoietic Stem and Progenitor Cells Acquire Distinct DNA-Hypermethylation During in vitro Culture. Scientific Reports. 2013 Nov 28;3:3372. Fulltext at PubMed*.

Hemeda H, Giebel B, Wagner W. Evaluation of Human Platelet Lysate versus Fetal Bovine Serum for Culture of Mesenchymal Stromal Cells. Cytotherapy. 2014 Feb;16(2):170-80.

Fuellen G, Boerries M, Busch H, de Grey AD, Hahn U, Hiller T, Hoeflich A, Jansen L, Janssens GE, Kaleta C, Meinema AC, Schäuble S, Schofield PN, Smith B, Sühnel J, Vera J, Wagner W, Wönne EC, Wuttke D, Simm A. In-Silico-Approaches and the Role of Ontologies in Aging Research. Rejuvenation Research, epub ahead of print.

Walenda G, Abnaof K, Joussen S, Meurer S, Smeets H, Rath B, Hoffmann K, Fröhlich H, Zenke M, Weiskirchen R, Wagner W. TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages. PLoS One. 2013 Oct 17;8(10):e77656. Fulltext at PubMed*.

Ludwig A, Saffrich R, Eckstein V, Bruckner T, Wagner W, Ho AD, Wuchter P. Functional potentials of human hematopoietic progenitor cells are maintained by mesenchymal stromal cells and not impaired by Plerixafor. Cytotherapy. 2014 Jan;16(1):111-21.

Hemeda H*, Kalz J*, Walenda G*, Lohmann M, Wagner W. Heparin concentration is critical for cell culture with human platelet lysate. Cytotherapy. 2013; 2013 Sep;15(9):1174-81.

Willmann CA, Hemeda H, Pieper LA, Lenz M, Qin J, Joussen S, Sontag S, Wanek P, Denecke B, Schüler HM, Zenke M, Wagner W. To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture. PLoS ONE. 2013; 8(5): e65324. Fulltext at PubMed*.

Koch CM, Wagner W. Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells. Methods in Molecular Biology. 2013; 1048: 309-321.

Lin Q, Wagner W, Zenke M. Analysis of human genome-wide DNA methylation profiles by BeadChip technology. Methods in Molecular Biology. 2013; 23(2): 248-259.

Koch CM, Reck K, Shao K, Lin Q, Joussen S, Ziegler P, Walenda G, Drescher W, Opalka B, May T, Brümmendorf T, Zenke M, Saric T, Wagner W. Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes. Genome Research. 2013; 23(2): 248-259.

Shao K, Koch CM, Gupta MK, Lin Q, Lenz L, Laufs S, Denecke B, Schmidt M, Linke M, Hennies HC, Hescheler J, Zenke M, Zechner U, Šarić T*, Wagner W* (2012). Induced Pluripotent Mesenchymal Stromal Cell Clones Retain Donor-Derived Differences in DNA Methylation Profiles. Molecular Therapy. 2013; 21(1): 240-250.

Ventura Ferreira MS, Schneider RK, Wagner W, Jahnen-Dechent W, Labude N, Bovi M, Piroth DM, Knüchel R, Hieronymus T, Müller AM, Zenke M, Neuss S. 2D Polymer-based Cultures expand Cord Blood-derived Hematopoietic Stem Cells and Support Engraftment of NSG mice. Tissue Eng Part C Methods, epub ahead of print.

Schellenberg A, Hemeda H, Wagner W. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency. Methods in Molecular Biology. 2013; 976: 143-154.

2012

Kramer J, Dazzi F, Dominici M, Schlenke P, Wagner W. Clinical Perspectives of Mesenchymal Stem Cells. Stem Cells International, 2012; Article ID 684827.

Wagner W. Implications of long-term culture for mesenchymal stem cells: genetic defects or epigenetic regulation? Stem Cell Research & Therapy. 2012; 3(6): 54.

Walenda G*, Hemeda H*, Schneider RK, Merkel R, Hoffmann B, Wagner W. Human Platelet Lysate Gel Provides a Novel 3D-Matrix for Enhanced Culture Expansion of Mesenchymal Stromal Cells. Tissue Engineering. 2012; 18(12): 924-934.

Zepeda-Moreno A, Saffrich R, Walenda T, Hoang VT, Wuchter P, Sánchez-Enríquez S, Corona-Rivera A, Wagner W and Ho AD. Modeling SDF-1 induced mobilization in leukemia cell lines. Experimental Hematology. 2012; 40(8): 666-674.

Lohmann M, Walenda G, Hemeda H, Joussen S, Drescher W, Jockenhoevel S, Hutschenreuter G, Zenke M and Wagner W. Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells. PLoS ONE. 2012; 7(5): e37839. Fulltext at PubMed*. (> 20 citations)

Ventura-Fereira M, Labude N, Walenda G, Adamzyk C, Wagner W, Pieroth D, Müller AM, Knüchel R, Hieronymus T, Zenke M, Jahnen-Dechent W, Neuss S. Ex vivo Expansion of Cord Blood-CD34+ Cells Using IGFBP2 and Angptl-5 Impairs Short-term Lymphoid Repopulation In vivo. J Tissue Eng and Reg Med; epub ahead of print.

Koch CM, Joussen S, Schellenberg A, Lin Q, Zenke M, Wagner W. Monitoring of cellular senescence by DNA-methylation at specific CpG sites. Aging Cell. 2012; 11(2): 366-369. (> 20 citations)

Bissels U, Bosio A, Wagner W. MicroRNAs are shaping the hematopoietic landscape. Haematologica. 2012; 97(2): 160-167. Free article*. (> 30 citations)

Leisten I, Kramann R, Ventura Ferreira MS, Bovi M, Neuss S, Ziegler P, Wagner W, Knüchel R, Schneider RK. Three-dimensional co-culture of hematopoietic stem and progenitor cells and mesenchymal stem cells in collagen scaffolds: An in vitro model of the hematopoetic niche. Biomaterials. 2012; 33(6): 1736-1747.

Schellenberg A, Stiehl T, Horn P, Joussen S, Pallua N, Ho AD, Wagner W. The composition of subpopulations in mesenchymal stromal cells changes during culture expansion. Cytotherapy. 2012; 14(4): 401-411.

2011

Koch C and Wagner W. Epigenetic-Aging-Signature to determine age in different tissues. Aging. 2011; 3: 1-10. Free article*. (> 30 citations)

Schellenberg A, Lin Q, Schueler H, Koch CM, Joussen S, Denecke B, Walenda G, Pallua N, Suschek CV, Zenke M, and Wagner W. Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks. Aging. 2011; 3(9): 873-888. Fulltext at PubMed*. (> 40 citations).

Diehlmann A, Bork S, Saffrich R, Veh RW, Wagner W, Derst C. K-ATP channels in mesenchymal stromal stem cells: strong up-regulation of Kir6.2 subunits upon osteogenic differentiation. Tissue and Cell. 2011; 43(5): 331-336.

Wirz S, Dietrich M, Flanagan TC, Bokermann G, Wagner W, Schmitz-Rode T, Jockenhoevel S. Influence of PDGF-AB on Tissue Development in Autologous Platelet-Rich Plasma Gels. Tissue Engineering. 2011; 17(13-14): 1891-1899.

Bauerschlag DO, Ammerpohl O, Bräutigam K, Schem C, Li Q, Weigel MT, Hilpert F, Arnold N, Maass N, Meinhold-Heerlein I, Wagner W. Progression-Free Survival in Ovarian Cancer is Reflected in Epigenetic DNA-Methylation Profiles. Oncology. 2011; 80(1-2): 12-20.

Walenda T, Bokermann G, Jost E, Galm O, Schellenberg A, Koch CM, Piroth DM, Drescher W, Brümmendorf TH, Wagner W. Serum after autologous transplantation stimulates proliferation and expansion of human hematopoietic progenitor cells. PLoS ONE. 2011; 6(3): e18012. Fulltext at PubMed*.

Walenda T*, Bokermann G*, Ventura Ferreira MS, Piroth DM, Hieronymus T, Neuss S, Zenke M, Ho AD, Müller AM, Wagner W. Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem and progenitor cells. Experimental Hematology. 2011; 39(6): 617-628. (> 30 citations)

Koch C*, Suschek CV*, Bork S, Lin Q, Görgens M, Joussen S, Pallua N, Ho AD, Zenke M, Wagner W. Specific age-associated DNA methylation changes in human dermal fibroblasts. PLoS ONE. 2011; 6(2): e16679. Fulltext at PubMed*. (> 40 citations).

Zepeda-Moreno A, Taubert I, Hellwig I, Pietsch L, Lakshmanan VK, Wagner W, Ho AD. Innovative method for quantification of cell-cell adhesion in 96 well plates. Cell Adhesion & Migration. 2011; 5(3): 215-219.

Cholewa D, Stiehl T, Schellenberg A, Bokermann G, Joussen S, Koch C, Walenda T, Pallua N, Marciniak-Czochra A, Suschek CV, Wagner W. Expansion of Adipose Mesenchymal Stromal Cells is affected by Human Platelet Lysate and Plating Density. Cell Transplantation. 2011; 20(9): 1409-1422. (> 20 citations)

Bork S, Horn P, Castoldi M, Hellwig I, Ho AD, Wagner W. Adipogenic Differentiation of Human Mesenchymal Stromal Cells is Down-Regulated by microRNA-369-5p and Up-Regulated by microRNA-371. Journal of Cellular Physiology. 2011; 226(9): 2226-2234. (> 30 citations)

Horn P, Bork S, Wagner W. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis. Methods in Molecular Biology. 2011; 698: 23-35.

2010

Wagner W. Senescence is heterogeneous in mesenchymal stromal cells - kaleidoscopes for cellular aging. Cell Cycle. 2010; 9(15): 2923-2924.

Horn P*, Bokermann G*, Cholewa D, Bork S, Walenda T, Koch C, Drescher W, Hutschenreuther G, Zenke M, Ho AD, Wagner W. Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy, 2010; 12(7): 888-898. (> 40 citations)

Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, Koch C. How to track aging of mesenchymal stromal cells? Aging. 2010; 2(4): 224-30. (> 50 citations)

Teschendorff AE, Menon U, Gentry-Maharaj A, Ramus SJ, Weisenberger DJ, Shen H, Campan M, Noushmehr H, Bell CG, Maxwell AP, Savage DA, Mueller-Holzner E, Marth C, Kocjan G, Gayther SA, Jones A, Beck S, Wagner W, Laird PW, Jacobs IJ, Widschwendter M. Age-dependent DNA methylation of genes that are suppressed in stem cells is a hallmark of cancer. Genome Res. 2010; 20(4): 440-446. (> 230 citations)

Wagner W, Ho AD, Zenke M. Different facets of aging in human mesenchymal stem cells. Tissue Engineering Part B Reviews. 2010; 16(4): 445-453. (> 70 citations)

Ong L., Li W., Oldigs J.K., Kaminski A., Gerstmayer B., Piechaczek C., Wagner W., Li R.K., Ma N. and Steinhoff G. Hypoxic/normoxic preconditioning increases endothelial differentiation potential of human bone marrow CD133+ cells. Tissue Eng Part C Methods. 2010; 16(5): 1069-1081. (> 20 citations)

Wein F, Pietsch L, Saffrich R, Wuchter P, Walenda T, Bork S, Horn P, Diehlmann A, Eckstein V, Ho AD, Wagner W. N-Cadherin is expressed on Human Hematopoietic Progenitor Cells and Mediates Interaction with Human Mesenchymal Stromal Cells. Stem Cell Research. 2010; 4(2): 129-139. (> 30 citations)

Schallmoser K*, Bartmann C*, Rohde E, Bork S, Guelly C, Obenauf AC, Reinisch A, Horn P, Ho AD, Strunk D and Wagner W. Replicative Senescence-Associated Gene Expression Changes in Mesenchymal Stromal Cells are Similar under Different Culture Conditions. Haematologica. 2010; 95(6): 867-874. Fulltext at PubMed*. (> 40 citations)

Bork S*, Pfister S*, Witt H, Horn P, Korn B, Ho AD, Wagner W. DNA Methylation Pattern Changes upon Long-Term Culture and Aging of Human Mesenchymal Stromal Cells. Aging Cell. 2010; 9(1): 54-63. Fulltext at PubMed*. (> 120 citations).

Walenda T, Bork S, Horn P, Wein F, Saffrich R, Diehlmann A, Eckstein V, Ho AD, Wagner W. Co-Culture with Mesenchymal Stromal Cells Increases Proliferation and Maintenance of Hematopoietic Progenitor Cells. Journal of Cellular and Molecular Medicine. 2010; 14(1-2): 337-350. (> 50 citations)

2009

Marciniak-Czochra A, Stiehl T, Wagner W. Modeling of replicative senescence in hematopoietic development. AGING. 2009; 1(8): 723-732. Fulltext at PubMed*. (> 20 citations).

Wagner W, Bork S, Horn P, Krunic D, Walenda T, Diehlmann A, Benes V, Blake J, Huber FX, Eckstein V, Boukamp P, Ho AD. Aging and replicative senescence have related effects on human stem and progenitor cells. PLoS One. 2009; 4(6):e5846. Fulltext at PubMed*. (> 150 citations)

Cao X, Pettit ME, Conlan SL, Wagner W, Ho AD, Clare AS, Callow JA, Callow ME, Grunze M, Rosenhahn A. Resistance of Polysaccharide Coatings to Proteins, Hematopoietic Cells, and Marine Organisms. Biomacromolecules. 2009; 10(4): 907-915. (> 20 citations)

Dickhut A, Pelttari K, Janicki P, Wagner W, Eckstein V, Egermann M, Richter W. Calcification or dedifferentiation: requirement to lock mesenchymal stem cells in a desired differentiation stage, J Cellular Physiol. 2009; 219: 219-226. (> 70 citations)

Marciniak-Czochra A, Stiehl T, Ho AD, Jäger W, Wagner W. Modelling of asymmetric cell division in hematopoietic stem cells - regulation of self-renewal is most essential for efficient repopulation. Stem Cells Dev. 2009; 18(3): 377-385. (> 60 citations).

2008

Horn P, Bork S, Diehlmann A, Walenda T, Eckstein V, Ho AD, Wagner W. Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy. 2008; 10: 676-685. (> 60 citations)

Nesselmann C, Ma N, Bieback K, Wagner W, Ho A, Konttinen YT, Zhang H, Hinescu ME, Steinhoff G. Mesenchymal stem cells and cardiac repair. Journal of Cellular and Molecular Medicine. 2008; 12: 1795-1810. (> 70 citations)

Beckermann BM, Kallifatidis G, Groth A, Frommhold D, Apel A, Mattern J, Salnikov AV, Moldenhauer G, Wagner W, Diehlmann A, Saffrich R, Schubert M, Ho AD, Giese N, Büchler MW, Friess H, Büchler P, Herr I. VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma. Br J Cancer. Br J Cancer. 2008; 19(99): 622-631. Fulltext at PubMed*. (> 150 citations)

Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R, Benes V, Blake J, Pfister S, Eckstein V, Ho AD. Replicative senescence of mesenchymal stem cells: a continuous and organized process. 2008; PLoS ONE. 21;3: e2213. Fulltext at PubMed*. (> 350 citations)

Wagner W, Horn P, Bork S, Ho AD. Aging of hematopoietic stem cells is regulated by the stem cell niche. Exp Gerontol. 2008; 43(11): 974-980. (> 60 citations)

Wagner W, Saffrich R, Ho AD. The stromal activity of mesenchymal stromal cells. Transfus Med and Hemotherapy. 2008; 35: 185-193. Fulltext at PubMed*.

Ho AD, Wagner W, Franke W. Heterogeneity of mesenchymal stem cell preparations. Cytotherapy. 2008; 10: 320-330. (> 120 citations)

Bürgers HF, Schelshorn DW, Wagner W, Kuschinsky W, Maurer MH. Acute anoxia stimulates proliferation in adult neural stem cells from the rat brain. Exp Brain Res. 2008; 188(1): 33-43. (> 30 citations)

Wagner W, Wein F, Roderburg C, Saffrich R, Diehlmann A, Eckstein V, Ho AD. Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44. Cells Tissues Organs. 2008; 188: 160-169. (> 30 citations)

2007

Wagner W, Roderburg C, Wein F, Diehlmann A, Frankhauser M, Schubert R, Eckstein V, Ho AD. Free article*. (> 110 citations)

Kallifatidis G, Beckermann B, Groth A, Schubert M, Apel A, A. Khamidjanov, E. Ryschich, Wenger T, Wagner W, Diehlmann A, Saffrich R, Eckstein V, Krause U, Mattern J, Chai M, Schütz G, Ho A, Gebhard MM, Büchler MW, Friess H, Büchler P, Herr I. Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer. Cancer Gene Therapy. 2007; 15: 231-240. (> 40 citations)

Wagner W, Ho AD. Mesenchymal stem cell preparations - comparing apples and oranges. Stem Cell Rev. 2007; 3: 239-248. (> 170 citations)

Ho AD, Wagner W. The beauty of asymmetry - asymmetric divisions and self-renewal in the hematopoietic system. Current Opinion in Hematology. 2007; 14: 330-336. (> 40 citations)

Faber A, Roderburg C, Wein F, Saffrich R, Seckinger A, Horsch K, Diehlmann A, Wong D, Bridger G, Eckstein V, Ho AD, Wagner W. The many facets of SDF-1alpha, CXCR4 agonists and antagonists on hematopoietic progenitor cells. Journal of Biomedicine Biotechnology. 2007; 3: 26065-26074. Fulltext at PubMed*. (> 30 citations)

Wagner W, Wein F, Roderburg C, Saffrich R, Faber A, Krause U, Schubert M, Benes V, Eckstein V, Maul H, and Ho AD. Adhesion of hematopoietic progenitor cells to human mesenchymal stromal cells as a model for cell-cell interaction. Experimental Hematology. 2007; 35: 314-325. (> 70 citations)

Molecular and secretory profiles of human mesenchymal stromal cells and their abilities to maintain "stemness" of hematopoietic progenitors. Stem Cells. 2007, 25: 2638-2647.

2006

Ho AD, Wagner W. Bone marrow niche and leukemia. Ernst Schering Found Symp Proc. 2006; 5: 125.39.

Fruehauf S., Seeger T., Maier P., Li L., Weinhardt S., Laufs S., Wagner W., Eckstein V., Bridger G., Calandra G., Wenz F., Zeller W. J., Goldschmidt H., Ho A. D. The CXCR4 antagonist AMD3100 releases a subset of G-CSF primed peripheral blood progenitor cells with specific gene expression characteristics. Experimental Hematology. 2006; 34, 1052-1059. (> 50 citations)

Wagner W., Feldmann Jr.R.E., Seckinger A, Maurer M.H., Wein F, Blake J, Krause U, Kalenka A, Bürgers H.F., Saffrich R, Wuchter P, Kuschinsky W, Ho A.D. The heterogeneity of human mesenchymal stem cell preparations - evidence from simultaneous analysis of proteomes and transcritpomes. Experimental Hematology. 2006; 34, 536-548. (> 120 citations)

2005

Ho AD, Wagner W, Mahlknecht U. Stem cells and ageing. The potential of stem cells to overcome age-related deteriorations of the body in regenerative medicine. EMBO Rep. 2005; 6: 35-38. Fulltext at PubMed*. (> 50 citations)

Wagner W, Saffrich R, Wirkner U, Eckstein V, Blake J, Ansorge A, Schwager C, Wein F, Miesala K, Ansorge W, and Ho AD. Hematopoietic progenitor cells and cellular microenvironment: behavioral and molecular changes upon interaction. Stem Cells. 2005; 23: 1180-1191. Free article*. (> 60 citations)

Wagner W, Laufs S, Blake J, Schwager C, Wu X, Zeller JW, Ho AD, Fruehauf S. Retroviral integration sites correlate with expressed genes in hematopoietic stem cells. Free article*.

Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, Blake J, Schwager C, Eckstein V, Ansorge W, Ho AD.

2004

Wagner W, Ansorge A, Wirkner U, Eckstein V, Schwager C, Blake J, Miesala K, Selig J, Saffrich R, Ansorge W, Ho AD. Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis. Blood. 2004; 104: 675-686. Free article*. (> 120 citations)

Book sections

Drescher W, Knobe M, Wagner W, Pufe T. New Therapies of Bone Necrosis; in K.-H. Koo et al.

Wuchter P, Wagner W, Eckstein V, Ho AD. Das Altern im Spiegelbild der Stammzellen - Irrwege und Auswege. In "Altern gestalten, Medizin, Technik, Umwelt". H. Häfner, K. Beyreuther, W. Schlicht (Eds), DOI 10.1007/e-ISBN, Springer Verlag, 2010.

(eds.), Osteonecrosis, Springer 2014, DOI 10.1007/978-3-642-35767-1_37, Heidelberg; 2014.

Wuchter P, Wagner W, Ho AD. Mesenchymal stem cells - an oversimplified nomenclature for extremely heterogenous progenitors. Springer textbook publication: Regenerative Medicine - From Protocol to Patient.

Ho AD, Wagner W, Eckstein V. Ein Mensch ist so alt wie seine Stammzellen.

Ho, AD, Wagner W. Bone marrow niche and leukemia.

Ho AD, Wagner W. Clinical potentials of stem cells -- hype or hope? In "Stem Cell Transplantation" Zanjani E, Ho AD (Eds). Wiley Press, ISBN-13: 978-3-527-31018-0, Weinheim; 2006.

Wirkner U, Maercker C, Abdollahi A, Wagner M, Selig J, Drzonek H, Radelof U, Kellermann A, Ansorge A, Maurer J, Schwager C, Korn B, Wagner W, Ho AD, Huber P, Ansorge W. Human genome on a chip in biomedical research. In "Molecular Diagnostics", Patrinos GP, Ansorge W. (Eds). Elsevier Science, ISBN-13: 978-0-12-546661-5, Amsterdam, Netherlands; 2004.

In "Was ist Alter(n). Neue Antworten auf eine scheinbar einfache Frage." Staudinger UM, Häfner H (Eds). Springer Verlag, ISBN978-3-540-76710-7, Heidelberg; 2008.In "Cancer Stem Cells -- Novel Concepts and Prospects for Tumor Therapy". Springer Verlag, ISBN 0947-6075, Heidelberg; 2007.

Dissertations

Walenda T. In vitro expansion of hematopoietic stem- and progenitor cells using components of the stem cell niche. Hochschulbibliothek der RWTH Aachen, 2012. Fulltext online available (German)*.

Walenda G. Optimization of culture conditions for the expansion of mesenchymal stromal cells. Hochschulbibliothek der RWTH Aachen, 2014. Fulltext online available (German)*.

Context Column



This research was funded by:

We would like explicitly to thank all patients who gave their consent to that we could use their cell samples for our research! Without this support, our work would not be possible.