Antigen presenting dendritic cells (DC) represent highly specialized immune cells with a central role in immunity and tolerance induction. DC sense antigens, which are taken-up, processed and presented in the context of MHC molecules to elicit antigen specific T cell responses. Specific DC subsets exist that differ in surface phenotype, function, activation state and anatomical localization, including (i) classical DC type 1 and 2 (cDC1 and cDC2, respectively) in lymphoid and non-lymphoid tissues; (ii) plasmacytoid DC (pDC) in blood that represent the major producers of type 1 interferon and (iii) Langerhans cells (LC), the cutaneous contingent of DC in epidermis.
Langerhans cells of epidermal skin are stained
for MHC class II (green)
The helix-loop-helix transcription factor Id2 represents a determining factor for DC development (Hacker et al., 2003; Zenke and Hieronymus, 2006; Seré et al., 2012). Id2-/- mice lack LC and cDC1. TGFbeta1-/- mice also lack LC and we show that TGFbeta1 acts upstream of Id2 and induces Id2 expression.
We identified two types of LC: short-term LC and long-term LC cells (Seré et al., 2012). Short-term LC develop from Gr-1+ monocytes under inflammatory conditions and are Id2-independent. Long-term LC arise from bone marrow under steady state and depend on Id2. LC reconstitution after inflammation occurs in two waves: an initial fast wave of Gr-1+ monocyte-derived short-term LC, which is followed by a second wave of bone marrow-derived long-term LC.
Hematopoietic stem cells and LC precursors in skin develop into long-term LC in steady state, which requires the transcription factor Id2 (A, top panel). In inflammation Gr-1+ monocytes develop into short-term LC, which does not require Id2 (A, lower panel). LC development in inflammation occurs in consecutive waves of short-term LC and long-term LC (B).
DC subsets develop from hematopoietic stem cells via Flt3 expressing progenitors through consecutive steps of lineage commitment and differentiation: multipotent progenitors (MPP) are committed to DC restricted common DC progenitors (CDP), which differentiate into the specific DC subsets cDC1 cDC2 and pDC. The laboratory studies gene expression and chromatin architecture of the MPP-CDP-cDC/pDC sequel by employing genome wide approaches with RNA-Seq, ChIP-Seq, ATAC-Seq and HiChIP-seq and a rich toolbox of bioinformatics (Hieronymus et al., 2005; Felker et al., 2010; Chauvistré et al., 2014; Lin et al., 2015; Gusmao et al., 2016; Li et al., 2019; in collaboration with Ivan Costa, Institute for Computational Genomics, RWTH Aachen University, Aachen, Germany).
Gene expression (mRNA), H3K4me1, H3K4me3, H3K27me3 and PU.1 occupancy of the sequel MPP-CDP-cDC/pDC (left). Histone and PU.1 profile of Flt3 gene (right; Lin et al., 2015).
Specific H3K4me1, H3K4me3 and H3K27me3 marks in CDP reveal a DC-primed epigenetic signature, which is maintained and reinforced during DC differentiation. We describe the circuitry of transcription factors, including Irf4, Irf8, Tcf4, Spib and Stats, which drives the sequel MPP-CDP-cDC/pDC. The circuitry also includes positive feedback loops inferred for individual or multiple factors that stabilize the distinct stages of DC development.
Network illustration of the integrated DC regulatory circuitry of the sequel MPP-CDP-cDC/pDC (Lin et al., 2015). Transcription factors that impact on DC development in gene knockout studies are indicated in red. Feedback loops that stabilize the distinct DC stages in the network are depicted (red arrows).
Data are available by the customized UCSC genome browser track data hub www.molcell.rwth-aachen.de/dc/.
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