Open positions at MOCA

Im Institut für Molekulare und Zelluläre Anatomie der Uniklinik RWTH Aachen sind zum frühestmöglichen Zeitpunkt folgende Stellen zu besetzen: 

We are currently seeking a highly motivated

Master student (f/m/d)

for a project on “ Regulation of cardiogenesis in desmosome deficient mouse embryos”.

Desmosomes are important structural junctions between cardiomyocytes that maintain cellular structure and confer tissue integrity. In the “Heart research group” of MOCA we aim to study the impact of mutations in desmosomal proteins during early heart morphogenesis. To this end, transgenic animal models will be utilized to investigate in vivo cardiogenesis under specific genetic conditions. In the first phase of the project, desmosome formation will be compared in different transgenic animals to wild type controls. In the second phase of the project cardiomyocytes shape, cytoarchitechture and their extracellular matrix composition will be studied.

Your Tasks

  • Histological analysis of embryonic heart, including tissue fixation, sectioning by microtome and staining of the tissue slides
  • Detection of protein expression and localization using immunohistochemical techniques
  • Visualizing protein expression and localization by fluorescence microscopy and analyzing data via ImageJ software.
  • Analysis of RNA expression and localization via in situ hybridization techniques

Your profile

  • B.Sc. in biology, biomedical  or related studies
  • Interested in developmental aspects of heart
  • Previous lab experiences is a plus but not required
  • Detail oriented, a good observer, and well organized

**Funding is available to support the student as a HIWI.

Please send your application including your CV, cover letter and transcripts to Mrs. Dr. Hoda Moazzen, hmoazzenukaachende.

Institute of Molecular and Cellular Anatomy
RWTH Aachen University

Wendlingweg 2
D-52057 Aachen
Phone: +49 (0) 241 80 85298
Email: hmoazzenukaachende
Web:  https://www.moca.rwth-aachen.de/heart_disease.html

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We are currently seeking a highly motivated

medical student (f/m/d)

for a project on “Mechanobiology of genetically modified retinal epithelium”.

The integrity and homeostasis of the retinal pigment epithelium (RPE) are critical to sustain the healthy function of the retina. RPE cells tightly interact with each other, forming a monolayer of cuboidal and polarized cells located between the choriocapillaris and the photoreceptor outer segments. It fulfils multiple tasks, including the maintenance of the blood-retina barrier to protect the retina, the transport of nutrients, the removal of metabolic products and the secretion of vital factors and molecules. Degeneration of the RPE interferes with the normal retinal metabolism, breaks the blood-retina barrier and causes vision loss. The RPE undergoes chronic mechanical stress, which generally plays a critical role in the (patho-)physiology of living cells. Dysfunctions contribute to the pathogenesis of many retinal degenerative diseases, such as proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), RPE tears and high myopia. These diseases have global prevalences of up to 3% and are characterized by harmful stretching of the RPE, resulting in the distortion of the retinal architecture up to retinal detachment and leading to the disruption of the essential interactions between RPE and outer retina and, finally, to blindness.

This project aims to characterize the mechanobiology of genetically modified retinal pigment epithelium. The genes for pigment epithelium-derived factor (PEDF) and brain-derived neurotrophic factor (BDNF) are delivered via electroporation using the Sleeping Beauty transposon system. The functionality of the monolayers from non-transfected and transfected cells will be performed by electrical impedance spectroscopy to monitor RPE barrier properties and morphological changes, as well as traction force microscopy and monolayer stress microscopy to evaluate RPE mechanobiological properties.

The resulting characterization of the genetically modified monolayers will set a step forward for the use of the transfection strategy in a novel therapeutic strategies.

Your Tasks

•    Optimization of traction force microscopy and electrical impedance spectroscopy of RPE.
•    Live imaging of RPE monolayer’s actin cytoskeleton with confocal microscopy
•    Computational data analyses using MATLAB and Fiji software

Your profile

•    You are studying medicine
•    You are interested in the field of mechanobiology
•    You are interested in mammalian cell culture
•    You are a reliable and careful worker with the ability of integrating well in a team

The student will have the possibility to apply for a scholarship to the German Society of Ophthalmology and will be working in a team of engineers, physicists, biologists and medical doctors from the groups of Dr. Di Russo (UKA, DWI), Dr. Johnen (UKA) and Dr. Linkhorst (RWTH).
If you are interested, please contact Dr. Jacopo Di Russo at jdirussoukaachende.

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We are currently seeking a highly motivated

Master student (f/m/d)

for a project on “Systematic characterization of cytoskeletal proteins of the human endometrium”.

The inner uterine wall (the so-called endometrium) undergoes hormone-driven cyclic changes to allow the embryo to implant; during the “window of implantation” on cycle days 19-23 the cell-cell junctions of endometrial cells remodel and only then the endometrium becomes receptive for the blastocyst. However, there are hardly any studies that look at these changes systematically. Through close cooperation with a fertility clinic, our institute has numerous endometrial biopsies. This allows us to characterize inter- and intraindividual cell morphological differences for the first time. This knowledge will contribute to a better understanding of the causes of involuntary childlessness.

Your Tasks

The goal of this thesis is the systematic characterization of relevant cytoskeletal proteins in the human endometrium during the „window of implantation“. You will learn how to embed human tissue in paraffin, slice it using a microtome, and label target structures by immunohistochemical techniques. Using modern microscopy techniques, you will learn how to detect, evaluate, and present antibody-labeled structures.

What we offer
  • A defined project that combines classical biomedical research methods with the clinics
  • Working in a methodically broadly based research institute and integration into the interdisciplinary grad school “Mechanobiology in 3D Epithelial Tissues (ME3T)“
  • Direct supervision with a high level of technical expertise in histology and microscopy
  • The possibility to bring in own ideas
  • Flexible start date and own office workstation

If you are interested, please contact Anna Sternberg, M.Sc., at asternbergkaachende.


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