Obesity and overweight can cause metabolic disorders. Frequent complications of obesity, changes in lipid metabolism in adipocytes. Normally, the amount of fatty acids released in the degradation is tightly regulated. Thus, the repertoire is different fatty acids in adipocytes and in the blood of an individual, unique and adapted to the current metabolic state. With increasing age, changes or slows the metabolism. In a clinical study, we want to look at the repertoire depends on fatty acids in adipose tissue and in the blood of patients by age and obesity. Adipose tissue contains except mainly mature fat cells and multipotent stem cells, and immune cells. Here we would like to examine how the age-related changes in fat cells influences the activation of immune cells surrounding.