We will pursue the following aims in myelofibrosis:

Aim 1. Dissect mechanisms of fibrotic transformation in myelofibrosis

Aim 2. Understand the changes leading to loss of normal hematopoiesis in myelofibrosis

Aim 3. Identify novel cellular and molecular targets in myelofibrosis

P1: Dissecting the bone marrow stromal contribution to myelofibrosis

P2: Interaction of the MPN clone and its microenvironment via the JAK1-STAT3 axis

P3: Dissecting mechanisms in the fibrotic reprogramming of the bone marrow niche in myelofibrosis

P4: The impact of human megakaryocytes in JAK2V617F and CALRmut induced myelofibrosis

P5: Cellular aging in myeloproliferative neoplasms

P6: Mechanisms of IFN-alpha (IFNa) in MPN-associated myelofibrosis

SP1: Transcriptional analysis in defined cell types and single cells